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antigen proteins  (Sino Biological)


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    Sino Biological antigen proteins
    Antigen Proteins, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd30/Human+CD30+%2F+TNFRSF8+Protein/pm39880067-318-8-15
    Average 91 stars, based on 3 article reviews
    antigen proteins - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Recombinant:

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Highly Potent, Anthracycline-based Antibody–Drug Conjugates Generated by Enzymatic, Site-specific Conjugation
    Article Snippet: For rodent sera, dilution series of ADC serum samples were captured on ELISA plates coated with 2 μg/mL of a mouse anti-PNU mAb (generated in-house by immunization of mice with Gly5-EDA-PNU-conjugated ADC) in order to bind PNU-conjugated mAb, or with goat anti-human Fc F(ab’)2 (Jackson Immunoresearch) in order to bind the ADC's mAb backbone, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG (Jackson). .. For primate sera, dilution series of ADC serum samples were captured on ELISA plates coated with 2 μg/mL of recombinant extracellular domain of human CD30 (Sino Biological) and detected with a 1:2500 dilution of HRP-conjugated donkey anti-human IgG (Jackson) to measure the Stefan et al. - 9 - concentration of the ADC's mAb backbone. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Highly Potent, Anthracycline-based Antibody–Drug Conjugates Generated by Enzymatic, Site-specific Conjugation
    Article Snippet: For rodent sera, dilution series of ADC serum samples were captured on ELISA plates coated with 2 μg/mL of a mouse anti-PNU mAb (generated in-house by immunization of mice with Gly5-EDA-PNU-conjugated ADC) in order to bind PNU-conjugated mAb, or with goat anti-human Fc F(ab’)2 (Jackson Immunoresearch) in order to bind the ADC's mAb backbone, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG (Jackson). .. For primate sera, dilution series of ADC serum samples were captured on ELISA plates coated with 2 μg/mL of recombinant extracellular domain of human CD30 (Sino Biological) and detected with a 1:2500 dilution of HRP-conjugated donkey anti-human IgG (Jackson) to measure the Stefan et al. - 9 - concentration of the ADC's mAb backbone. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Produced:

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Article Title: Binding protein drug conjugates comprising anthracycline derivatives
    Article Snippet: For rodent sera, dilution series of cAc10-PNU-EDA-Gly5 serum samples were captured on ELISA plates coated with 2 μg/ml of a mouse anti-PNU mAb (produced in-house by immunizing mice with a human IgG-PNU conjugate and screening with a BSA-PNU conjugate) to bind ADC, or with anti-human Fc F(ab′)2 (Jackson Immunoresearch) to bind total IgG, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG F(ab′)2 (Jackson Immunoresearch). .. For primate sera, 2 μg/ml of recombinant human CD30 (Sino Biologicals, 10777-H08H) was coated on ELISA plates and a 1:2500 dilution of HRP-conjugated anti-human IgG F(ab)2 (Jackson Immunoresearch) or 1 μg/ml of a mouse anti-PNU IgG (produced in-house) followed by HRP-conjugated anti-mouse Fc F(ab′)2 (Jackson Immunoresearch) was used for detection of total IgG and ADC, respectively. ..

    Incubation:

    Article Title: Development of chimeric Nanobody-Granzyme B functionalized ferritin nanoparticles for precise tumor therapy.
    Article Snippet: .. The coated plates were subsequently incubated with various antigen proteins (1 μg/mL), including human CD30 (Sino Biological, 10777-MM01–50), CD5 (Sino Biological, 11027-H08H), FGFR4 (Sino Biological, 10538-H08H), CD71 (Sino Biological, 11020-H07H-B-20), and 2 % BSA (negative control), at 200 μL/ well for 1 hour at 37◦C. .. This was followed by incubation with mouse anti-human antigen monoclonal antibodies (1:1000) for human CD30, CD5, FGFR4, and CD71 at 200 μL/well for 1 hour at 37◦C, and then with goat anti-mouse IgG H&L (HRP) (Abcam, ab6789) (1:10000) at 100 μL/ well for 1 hour at 37◦C.

    Negative Control:

    Article Title: Development of chimeric Nanobody-Granzyme B functionalized ferritin nanoparticles for precise tumor therapy.
    Article Snippet: .. The coated plates were subsequently incubated with various antigen proteins (1 μg/mL), including human CD30 (Sino Biological, 10777-MM01–50), CD5 (Sino Biological, 11027-H08H), FGFR4 (Sino Biological, 10538-H08H), CD71 (Sino Biological, 11020-H07H-B-20), and 2 % BSA (negative control), at 200 μL/ well for 1 hour at 37◦C. .. This was followed by incubation with mouse anti-human antigen monoclonal antibodies (1:1000) for human CD30, CD5, FGFR4, and CD71 at 200 μL/well for 1 hour at 37◦C, and then with goat anti-mouse IgG H&L (HRP) (Abcam, ab6789) (1:10000) at 100 μL/ well for 1 hour at 37◦C.

    Concentration Assay:

    Article Title: Highly Potent, Anthracycline-based Antibody–Drug Conjugates Generated by Enzymatic, Site-specific Conjugation
    Article Snippet: For rodent sera, dilution series of ADC serum samples were captured on ELISA plates coated with 2 μg/mL of a mouse anti-PNU mAb (generated in-house by immunization of mice with Gly5-EDA-PNU-conjugated ADC) in order to bind PNU-conjugated mAb, or with goat anti-human Fc F(ab’)2 (Jackson Immunoresearch) in order to bind the ADC's mAb backbone, and detected with a 1:2500 dilution of an HRP-conjugated anti-human IgG (Jackson). .. For primate sera, dilution series of ADC serum samples were captured on ELISA plates coated with 2 μg/mL of recombinant extracellular domain of human CD30 (Sino Biological) and detected with a 1:2500 dilution of HRP-conjugated donkey anti-human IgG (Jackson) to measure the Stefan et al. - 9 - concentration of the ADC's mAb backbone. ..



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    TG-1 suppresses <t>CD30-CD30L-mediated</t> B-NHL proliferation by targeting the non-canonical NF-κB pathway. Relative cell viability of Jeko-1 ( A ) and Raji ( B ) cells was assessed following incubation with 5 and 10 µg/ml of <t>CD30,</t> 10 µg/ml of CD30 pretreated with 25 µM TG-1, or 10 µg/ml of CD30 pretreated with 1 µg/ml CD30L antibodies for 4–6 h at 37 °C, as determined by MTT assays. ( C ) Relative cell viability of Granta-519 cells was evaluated after exposure to 10 µg/ml of CD30 that had been pretreated with the CD30L plasmid (1 µg), as well as with CD30 alone, using MTT assays. The expression levels of NFκB-2, IKKα, phosphorylated IKKα, and RELB in Jeko-1 ( D ) and Raji ( E ) cells were evaluated using Western blot analysis after treatment with 10 µg/ml CD30, preceded by pretreatment with either 25 µM TG-1 or1 µg/ml CD30L antibodies. ( F , G ) The protein expression levels of NFκB-2, IKKα, phosphorylated IKKα, and RELB in D and E were quantified using ImageJ. GAPDH served as an internal control. ( H-O ) Immunofluorescence was used to measure the expression levels of NFκB-2 and RELB in Jeko-1 ( H and I ) and Raji ( L and M ) cells following treatment with 10 µg/ml CD30, with a preceding pretreatment of either 25 µM TG-1 or1 µg/ml CD30L antibodies. Scale bar = 20 μm. ( J , K , N , and O ) The normalized fluorescence intensity for H , I , L , and M , respectively. ( P-S ) The relative cell viability of Jeko-1 ( P ), Raji ( Q ), Su-4 ( R ), and Granta-519 ( S ) cells was evaluated after exposure to combinations of 10 µg/ml CD30 and 200 nM IKK16, as determined by MTT assays. Data shown in this figure are the mean values (± SD) from three independent experiments. Statistically significant differences with P < 0.05 were considered significant (# P ≥ 0 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001)
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    R&D Systems cytometry application
    SASP Mediators in Jurkat cell line supernatants. Jurkat Tet off, Tat 72 or Tat 101, cells were grown in round bottom 96-well plates at 5×10 5 cells/ml in 200 μl R10, and cell supernatants were collected after 72 hours. The expression levels of 105 cytokines were addressed with an antibody-based membrane array. (A) Heatmap indicates the mean intensity values of the 43 mediators with visible spots in the membrane after ELC incubation. Mediators with mean signals over 1.4 (green boxes) or below 0.7-fold change (red boxes) expression in Tat 101 over Tet off (dotted line) are shown in (B) . Changes in Tat 72 over Tet off (C) and Tat 101 over Tat 72 (D) are also shown. (n=3). Secreted levels of <t>CD30</t> (E) and CD31 (F) were evaluated in Tet off (n=4), Tat 72 (n=2-3) and Tat 101 by Luminex on the same cell supernatants as (A) . The percentage of cells expressing cell surface CD30 (G) or CD31 (H) was addressed by Flow Cytometry. Graphs indicate percentages of cells within the Live cell gate after 48h cell culture. CD30 and CD31 co-expression for each of the cell lines (blue contour plot) overlayed with autofluorescence controls (grey contour plot) from a representative experiment is shown in (I) . Symbols represent individual experiments (n=3), and floating bars indicate minimum to maximum values with a line at the mean. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
    Cytometry Application, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    14d3 fluidigm 3161004b 2651711 169tm cd30  (fluidigm)
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    fluidigm 14d3 fluidigm 3161004b 2651711 169tm cd30
    SASP Mediators in Jurkat cell line supernatants. Jurkat Tet off, Tat 72 or Tat 101, cells were grown in round bottom 96-well plates at 5×10 5 cells/ml in 200 μl R10, and cell supernatants were collected after 72 hours. The expression levels of 105 cytokines were addressed with an antibody-based membrane array. (A) Heatmap indicates the mean intensity values of the 43 mediators with visible spots in the membrane after ELC incubation. Mediators with mean signals over 1.4 (green boxes) or below 0.7-fold change (red boxes) expression in Tat 101 over Tet off (dotted line) are shown in (B) . Changes in Tat 72 over Tet off (C) and Tat 101 over Tat 72 (D) are also shown. (n=3). Secreted levels of <t>CD30</t> (E) and CD31 (F) were evaluated in Tet off (n=4), Tat 72 (n=2-3) and Tat 101 by Luminex on the same cell supernatants as (A) . The percentage of cells expressing cell surface CD30 (G) or CD31 (H) was addressed by Flow Cytometry. Graphs indicate percentages of cells within the Live cell gate after 48h cell culture. CD30 and CD31 co-expression for each of the cell lines (blue contour plot) overlayed with autofluorescence controls (grey contour plot) from a representative experiment is shown in (I) . Symbols represent individual experiments (n=3), and floating bars indicate minimum to maximum values with a line at the mean. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
    14d3 Fluidigm 3161004b 2651711 169tm Cd30, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd30/Anti-Human+CD152%2FCTLA-4+(14D3)-161Dy/pmc11599746__41467_2024_54684_MOESM1_ESM-152-258-259
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    SASP Mediators in Jurkat cell line supernatants. Jurkat Tet off, Tat 72 or Tat 101, cells were grown in round bottom 96-well plates at 5×10 5 cells/ml in 200 μl R10, and cell supernatants were collected after 72 hours. The expression levels of 105 cytokines were addressed with an antibody-based membrane array. (A) Heatmap indicates the mean intensity values of the 43 mediators with visible spots in the membrane after ELC incubation. Mediators with mean signals over 1.4 (green boxes) or below 0.7-fold change (red boxes) expression in Tat 101 over Tet off (dotted line) are shown in (B) . Changes in Tat 72 over Tet off (C) and Tat 101 over Tat 72 (D) are also shown. (n=3). Secreted levels of <t>CD30</t> (E) and CD31 (F) were evaluated in Tet off (n=4), Tat 72 (n=2-3) and Tat 101 by Luminex on the same cell supernatants as (A) . The percentage of cells expressing cell surface CD30 (G) or CD31 (H) was addressed by Flow Cytometry. Graphs indicate percentages of cells within the Live cell gate after 48h cell culture. CD30 and CD31 co-expression for each of the cell lines (blue contour plot) overlayed with autofluorescence controls (grey contour plot) from a representative experiment is shown in (I) . Symbols represent individual experiments (n=3), and floating bars indicate minimum to maximum values with a line at the mean. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
    Goat Polyclonal Anti Cd30, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 3. <t>CD30</t> lateral flow assay. (A) CD30 standards were diluted from 0 to 10 ng/mL in a benign seroma. (B) Correlation of CD30 concentration with TL/CL ratio (r2 = 0.72, y = 0.05x + 0.7, P = .02). (C) Comparison of CD30 in benign and ALCL seromas (benign, 0.198 ± 0.036 [n = 15] vs ALCL, 0.779 ± 0.083 [n = 11]; P < .0001, t-test). CL, control line; TL, test line.
    Mouse Anti Cd30 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    TG-1 suppresses CD30-CD30L-mediated B-NHL proliferation by targeting the non-canonical NF-κB pathway. Relative cell viability of Jeko-1 ( A ) and Raji ( B ) cells was assessed following incubation with 5 and 10 µg/ml of CD30, 10 µg/ml of CD30 pretreated with 25 µM TG-1, or 10 µg/ml of CD30 pretreated with 1 µg/ml CD30L antibodies for 4–6 h at 37 °C, as determined by MTT assays. ( C ) Relative cell viability of Granta-519 cells was evaluated after exposure to 10 µg/ml of CD30 that had been pretreated with the CD30L plasmid (1 µg), as well as with CD30 alone, using MTT assays. The expression levels of NFκB-2, IKKα, phosphorylated IKKα, and RELB in Jeko-1 ( D ) and Raji ( E ) cells were evaluated using Western blot analysis after treatment with 10 µg/ml CD30, preceded by pretreatment with either 25 µM TG-1 or1 µg/ml CD30L antibodies. ( F , G ) The protein expression levels of NFκB-2, IKKα, phosphorylated IKKα, and RELB in D and E were quantified using ImageJ. GAPDH served as an internal control. ( H-O ) Immunofluorescence was used to measure the expression levels of NFκB-2 and RELB in Jeko-1 ( H and I ) and Raji ( L and M ) cells following treatment with 10 µg/ml CD30, with a preceding pretreatment of either 25 µM TG-1 or1 µg/ml CD30L antibodies. Scale bar = 20 μm. ( J , K , N , and O ) The normalized fluorescence intensity for H , I , L , and M , respectively. ( P-S ) The relative cell viability of Jeko-1 ( P ), Raji ( Q ), Su-4 ( R ), and Granta-519 ( S ) cells was evaluated after exposure to combinations of 10 µg/ml CD30 and 200 nM IKK16, as determined by MTT assays. Data shown in this figure are the mean values (± SD) from three independent experiments. Statistically significant differences with P < 0.05 were considered significant (# P ≥ 0 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001)

    Journal: Molecular Cancer

    Article Title: Targeting CD30L in B-cell non-Hodgkin lymphoma: novel peptide conjugates and their therapeutic potential

    doi: 10.1186/s12943-025-02393-9

    Figure Lengend Snippet: TG-1 suppresses CD30-CD30L-mediated B-NHL proliferation by targeting the non-canonical NF-κB pathway. Relative cell viability of Jeko-1 ( A ) and Raji ( B ) cells was assessed following incubation with 5 and 10 µg/ml of CD30, 10 µg/ml of CD30 pretreated with 25 µM TG-1, or 10 µg/ml of CD30 pretreated with 1 µg/ml CD30L antibodies for 4–6 h at 37 °C, as determined by MTT assays. ( C ) Relative cell viability of Granta-519 cells was evaluated after exposure to 10 µg/ml of CD30 that had been pretreated with the CD30L plasmid (1 µg), as well as with CD30 alone, using MTT assays. The expression levels of NFκB-2, IKKα, phosphorylated IKKα, and RELB in Jeko-1 ( D ) and Raji ( E ) cells were evaluated using Western blot analysis after treatment with 10 µg/ml CD30, preceded by pretreatment with either 25 µM TG-1 or1 µg/ml CD30L antibodies. ( F , G ) The protein expression levels of NFκB-2, IKKα, phosphorylated IKKα, and RELB in D and E were quantified using ImageJ. GAPDH served as an internal control. ( H-O ) Immunofluorescence was used to measure the expression levels of NFκB-2 and RELB in Jeko-1 ( H and I ) and Raji ( L and M ) cells following treatment with 10 µg/ml CD30, with a preceding pretreatment of either 25 µM TG-1 or1 µg/ml CD30L antibodies. Scale bar = 20 μm. ( J , K , N , and O ) The normalized fluorescence intensity for H , I , L , and M , respectively. ( P-S ) The relative cell viability of Jeko-1 ( P ), Raji ( Q ), Su-4 ( R ), and Granta-519 ( S ) cells was evaluated after exposure to combinations of 10 µg/ml CD30 and 200 nM IKK16, as determined by MTT assays. Data shown in this figure are the mean values (± SD) from three independent experiments. Statistically significant differences with P < 0.05 were considered significant (# P ≥ 0 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001)

    Article Snippet: Treatments included 25 μM TG-1 or Random peptides, recombinant human CD30 protein (HY-P70023, MedChemExpress, NJ, USA) at varying concentrations, and IKK16 (0-500 nM; HY-13687, ChemExpress, China), either alone or in combination, for 24 h at 37 °C.

    Techniques: Incubation, Plasmid Preparation, Expressing, Western Blot, Control, Immunofluorescence, Fluorescence

    Schematic illustration of the interaction between TG-1-DKK, F-TG-1-AuNPs, and F-TG-1-AuNPs + DOX with CD30L-positive B-NHL cells. Our in vitro experiments have shown that CD30 interacts with CD30L on B-NHL cells, resulting in the promotion of B cell proliferation and the suppression of cell death. Nanoparticles functionalized with the TG-1 peptide selectively bind to CD30L. This binding disrupts the CD30-CD30L interaction, inhibiting CD30L-mediated reverse signaling and leading to a decrease in proliferation. Furthermore, the conjugation of TG-1 with the anti-cancer peptide DKK enhances cell lysis by targeting CD30L. In vivo investigations have demonstrated that FITC-labeled TG-1 can identify and inhibit tumor cell proliferation through the bloodstream. Additionally, F-TG-1-AuNPs + DOX effectively inhibit tumor growth in vivo

    Journal: Molecular Cancer

    Article Title: Targeting CD30L in B-cell non-Hodgkin lymphoma: novel peptide conjugates and their therapeutic potential

    doi: 10.1186/s12943-025-02393-9

    Figure Lengend Snippet: Schematic illustration of the interaction between TG-1-DKK, F-TG-1-AuNPs, and F-TG-1-AuNPs + DOX with CD30L-positive B-NHL cells. Our in vitro experiments have shown that CD30 interacts with CD30L on B-NHL cells, resulting in the promotion of B cell proliferation and the suppression of cell death. Nanoparticles functionalized with the TG-1 peptide selectively bind to CD30L. This binding disrupts the CD30-CD30L interaction, inhibiting CD30L-mediated reverse signaling and leading to a decrease in proliferation. Furthermore, the conjugation of TG-1 with the anti-cancer peptide DKK enhances cell lysis by targeting CD30L. In vivo investigations have demonstrated that FITC-labeled TG-1 can identify and inhibit tumor cell proliferation through the bloodstream. Additionally, F-TG-1-AuNPs + DOX effectively inhibit tumor growth in vivo

    Article Snippet: Treatments included 25 μM TG-1 or Random peptides, recombinant human CD30 protein (HY-P70023, MedChemExpress, NJ, USA) at varying concentrations, and IKK16 (0-500 nM; HY-13687, ChemExpress, China), either alone or in combination, for 24 h at 37 °C.

    Techniques: In Vitro, Binding Assay, Conjugation Assay, Lysis, In Vivo, Labeling

    Spatial transcriptomics reveals pHL tumor microenvironment comparments and checkpoint blockade targets: A. Characterisation of Hodgkin samples by spatial transcriptomics. (Left) Integration, dimension reduction and unsupervised clustering of Visium spatial dots. (Middle) module scores distribution across clusters. (violin plot from left: Hodgkin, Histiocyte, M2 macrophage-like, B cell). (Right) cell distribution across each sample for different clusters visualised as heat map B. Representative Visium slide highlighting locations of clusters of interest (left, cluster3, 10 and 11) and contour plot of module scores calculated by pathologist curated gene sets. Hodgkin inclusive (red contour): TNFRSF8 (CD30), FUT4 (CD15), IRF4 ; Hodgkin exclusive (green contour): MS4A1 (CD20), CD79A , CD19 , SLC22A2 , POU2AF1 ; Exhaustion (blue contour): CTLA4 , LAG3 , TIGIT . C. Selected spatial gene expression of Hodgkin sample. D. Differently expressed gene (DEG) focused on extracellular signalling. (Top row) ligand (in red) or receptor (in blue) transcript upregulated in cluster of interest (from left: cluster 3, 10 & 11). (Bottom row) CellChat visualisation of selected top ligand-receptor pair from above. E. Selected gene expression of Visium clusters categorized into surface expression (cluster 3 only), progenitor B, Hodgkin markers, selected T cell immune check point expression and metalloproteins.

    Journal: bioRxiv

    Article Title: Single cell analysis reveals molecular traits of pediatric lymphoma resistant subclones

    doi: 10.64898/2026.04.21.719850

    Figure Lengend Snippet: Spatial transcriptomics reveals pHL tumor microenvironment comparments and checkpoint blockade targets: A. Characterisation of Hodgkin samples by spatial transcriptomics. (Left) Integration, dimension reduction and unsupervised clustering of Visium spatial dots. (Middle) module scores distribution across clusters. (violin plot from left: Hodgkin, Histiocyte, M2 macrophage-like, B cell). (Right) cell distribution across each sample for different clusters visualised as heat map B. Representative Visium slide highlighting locations of clusters of interest (left, cluster3, 10 and 11) and contour plot of module scores calculated by pathologist curated gene sets. Hodgkin inclusive (red contour): TNFRSF8 (CD30), FUT4 (CD15), IRF4 ; Hodgkin exclusive (green contour): MS4A1 (CD20), CD79A , CD19 , SLC22A2 , POU2AF1 ; Exhaustion (blue contour): CTLA4 , LAG3 , TIGIT . C. Selected spatial gene expression of Hodgkin sample. D. Differently expressed gene (DEG) focused on extracellular signalling. (Top row) ligand (in red) or receptor (in blue) transcript upregulated in cluster of interest (from left: cluster 3, 10 & 11). (Bottom row) CellChat visualisation of selected top ligand-receptor pair from above. E. Selected gene expression of Visium clusters categorized into surface expression (cluster 3 only), progenitor B, Hodgkin markers, selected T cell immune check point expression and metalloproteins.

    Article Snippet: The sections were stained with CD30 (#BAF1028, R&D systems), CD3 (#300415, Biolegend), PD1 (#AB201825, Abcam), LAG3 (#AB270908, Abcam), LMNA (#AB193903, Abcam), DAPI and mounted with ProLong glass antifade Mountant (#P36984, Invitrogen).

    Techniques: Spatial Transcriptomics, Gene Expression, Expressing

    SASP Mediators in Jurkat cell line supernatants. Jurkat Tet off, Tat 72 or Tat 101, cells were grown in round bottom 96-well plates at 5×10 5 cells/ml in 200 μl R10, and cell supernatants were collected after 72 hours. The expression levels of 105 cytokines were addressed with an antibody-based membrane array. (A) Heatmap indicates the mean intensity values of the 43 mediators with visible spots in the membrane after ELC incubation. Mediators with mean signals over 1.4 (green boxes) or below 0.7-fold change (red boxes) expression in Tat 101 over Tet off (dotted line) are shown in (B) . Changes in Tat 72 over Tet off (C) and Tat 101 over Tat 72 (D) are also shown. (n=3). Secreted levels of CD30 (E) and CD31 (F) were evaluated in Tet off (n=4), Tat 72 (n=2-3) and Tat 101 by Luminex on the same cell supernatants as (A) . The percentage of cells expressing cell surface CD30 (G) or CD31 (H) was addressed by Flow Cytometry. Graphs indicate percentages of cells within the Live cell gate after 48h cell culture. CD30 and CD31 co-expression for each of the cell lines (blue contour plot) overlayed with autofluorescence controls (grey contour plot) from a representative experiment is shown in (I) . Symbols represent individual experiments (n=3), and floating bars indicate minimum to maximum values with a line at the mean. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

    Journal: Frontiers in Immunology

    Article Title: HIV-Tat upregulates the expression of senescence biomarkers in CD4 + T-cells

    doi: 10.3389/fimmu.2025.1568762

    Figure Lengend Snippet: SASP Mediators in Jurkat cell line supernatants. Jurkat Tet off, Tat 72 or Tat 101, cells were grown in round bottom 96-well plates at 5×10 5 cells/ml in 200 μl R10, and cell supernatants were collected after 72 hours. The expression levels of 105 cytokines were addressed with an antibody-based membrane array. (A) Heatmap indicates the mean intensity values of the 43 mediators with visible spots in the membrane after ELC incubation. Mediators with mean signals over 1.4 (green boxes) or below 0.7-fold change (red boxes) expression in Tat 101 over Tet off (dotted line) are shown in (B) . Changes in Tat 72 over Tet off (C) and Tat 101 over Tat 72 (D) are also shown. (n=3). Secreted levels of CD30 (E) and CD31 (F) were evaluated in Tet off (n=4), Tat 72 (n=2-3) and Tat 101 by Luminex on the same cell supernatants as (A) . The percentage of cells expressing cell surface CD30 (G) or CD31 (H) was addressed by Flow Cytometry. Graphs indicate percentages of cells within the Live cell gate after 48h cell culture. CD30 and CD31 co-expression for each of the cell lines (blue contour plot) overlayed with autofluorescence controls (grey contour plot) from a representative experiment is shown in (I) . Symbols represent individual experiments (n=3), and floating bars indicate minimum to maximum values with a line at the mean. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

    Article Snippet: For absolute quantification of sCD31, and sCD30 levels in cell-supernatants, a CD31 (PECAM-1) Human ProcartaPlexTM Simplex Kit, and a CD30 Human ProcartaPlexTM Simplex Kit were used, following the manufacturer’s instructions (Thermo Fisher Scientific).

    Techniques: Expressing, Membrane, Incubation, Luminex, Flow Cytometry, Cell Culture

    Figure 3. CD30 lateral flow assay. (A) CD30 standards were diluted from 0 to 10 ng/mL in a benign seroma. (B) Correlation of CD30 concentration with TL/CL ratio (r2 = 0.72, y = 0.05x + 0.7, P = .02). (C) Comparison of CD30 in benign and ALCL seromas (benign, 0.198 ± 0.036 [n = 15] vs ALCL, 0.779 ± 0.083 [n = 11]; P < .0001, t-test). CL, control line; TL, test line.

    Journal: Aesthetic surgery journal

    Article Title: IL-9 Is a Biomarker of BIA-ALCL Detected Rapidly by Lateral Flow Assay.

    doi: 10.1093/asj/sjae137

    Figure Lengend Snippet: Figure 3. CD30 lateral flow assay. (A) CD30 standards were diluted from 0 to 10 ng/mL in a benign seroma. (B) Correlation of CD30 concentration with TL/CL ratio (r2 = 0.72, y = 0.05x + 0.7, P = .02). (C) Comparison of CD30 in benign and ALCL seromas (benign, 0.198 ± 0.036 [n = 15] vs ALCL, 0.779 ± 0.083 [n = 11]; P < .0001, t-test). CL, control line; TL, test line.

    Article Snippet: Similarly, CD30 LFA strips were made by striping mouse anti-CD30 antibody (R&D Systems, catalog number MAB2291).

    Techniques: Lateral Flow Assay, Concentration Assay, Comparison, Control